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The CDC Biofilm Reactor was developed to provide consistent biofilm samples and growth conditions for evaluation of antimicrobial agents, surface treatments and materials. As described in ASTM 2562-07: “Standard Test Method for Quantification of Pseudomonas aeruginosa Grown with High Shear and Continuous Flow using a CDC Biofilm Reactor”.

The CDC Biofilm Reactor consists of eight (8) polypropylene coupon holders suspended from a UHMW-polyethylene ported lid. The coupon holders can accommodate 3 x1/2 inch (12.7 mm) diameter coupons each. The lid with coupon holders and coupons is mounted in a 1 liter glass vessel with side-arm discharge port. A liquid growth media/biocide/etc. is circulated through the vessel while mixing and shear is generated by a magnetic stir bar/vane rotated by a magnetic stir plate.

Sampling of the coupons is conducted by aseptically removing individual coupon holders with accompanying coupons (3). The coupon holder or a stopper is replaced in the lid after sampling to allow the time course experiment to continue.

The coupon removed from the reactor vessel is then used for treatment evaluations, scraped to collect the biofilm sample for further study, or imaging using microscopy and image analysis.

RD128 Coupons are available from a wide range of materials including plastics, metals, ceramics, glass, and rubber to fit the CDC Biofilm Reactor, Rotating Disc Reactor, and FC 71 Flow Cells.

Specs for the CDC Biofilm Reactor

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